Molecular Biology:

Molecular Biology is dedicated to supporting scientific exploration in the fields of molecular

biology, genomics, and genetic engineering. The facility is equipped with essential instrumentation for

genetic sequencing, gene expression analysis, DNA cloning, as well as the synthesis of oligonucleotides

(primers) crucial for various molecular biology applications.

DNA-oligonucleotide synthesizer ASM-800:

The ASM-800 synthesizer streamlines the oligonucleotide synthesis process, allowing researchers to produce sequences of varying lengths quickly and accurately. It utilizes solid-phase synthesis techniques, controlled chemical reactions, and specialized reagents to assemble nucleotide building blocks into custom-designed oligonucleotides.

Features:

• Efficiency: 8 oligonucleotides in 4 hours

• Synthesis scales: 0.04 – 0.4 µmol

• Length of oligonucleotides: up to 249 mer

• Coupling efficiency: more than 98.5% (DMTr)

• Operation trityl monitoring

• Working gas: Helium.

Applied Biosystems ABI 3500 Genetic DNA Analyzer:

The 3500 analyzer is a fluorescence-based DNA analysis system that uses proven capillary electrophoresis technology with 8 capillaries. The 3500 analyzer is fully automated, from sample loading to primary data analysis, for sequencing and fragment analysis.

Features:

• Capillary Length 50 cm, 36 cm

• Format 96-well plate, 8-well strips

• Number of Capillaries 8 Capillaries

• Polymer POP-4™, POP-6™, POP-7™

• Laser Features Long-life single-line 505 nm solid-state laser.

Bioanalyzer 2100 Agilent:

The 2100 Bioanalyzer system is an automated electrophoresis tool for the sample quality control of biomolecules. The 2100 Bioanalyzer employs a microfluidics-based electrophoresis technique to separate and analyze nucleic acids and proteins. This method allows for precise sizing, quantification, and quality assessment of biomolecules. It is used for Nucleic Acid Analysis: the assessment of DNA and RNA integrity, quantification of nucleic acid samples, and sizing of DNA fragments, such as PCR products and DNA libraries for sequencing.

Real Time PCR (CFX96, Bio-Rad):

The CFX96 Real-Time PCR System from Bio-Rad is a powerful tool used for quantitative PCR (qPCR) to monitor DNA or RNA amplification in real-time. With its 96-well format, it can process multiple samples simultaneously, making it ideal for gene expression analysis, genotyping, and pathogen detection. The system is equipped with advanced fluorescence detection capabilities, allowing for high sensitivity and the ability to multiplex, tracking multiple targets in a single run. Its intuitive software offers comprehensive data analysis, making it a go-to instrument for both research and clinical labs.