Flow Cytometry:

Our Flow Cytometry facility allows the examination of cells and particles in fluid suspension. Researchers can precisely measure and sort individual cells based on multiple parameters such as size, shape, and fluorescence intensity. This enables a deeper understanding of cellular populations, immune responses, and the study of complex diseases at the single-cell level. Flow cytometry applications

• Apoptosis.

• Cell cycle analysis.

• Multi-color flow cytometric analysis.

• Immunophenotyping.

• Sorting of target cells.

Flow cytometer Attune Nxt (Thermo Fisher):

Excitation lasers - violet (405 nm), blue (488nm), yellow (561nm), red (637nm).

Sample type – cell suspension.
Sample analysis volume: 200 μL to 4 mL.
Custom sample flow rates: 12.5–1,000 μL/min.
Fluorescence Sensitivity: 80 MESF FITC, 30 MESF PE, 70 MESF APC.

Cell Sorter MA900 (Sony Biotechnology):

Excitation lasers - violet (405 nm), blue (488nm), yellow (561nm), red (638nm).
Scatter resolution 0.5 µm.

Sample type – cell suspension.
Tube types -  0.5-mL, 1.5-mL, 5-mL and 15-mL tubes.
Sorting chip size -  70 μm, 100 μm, 130 μm.
Sort Devices - 2-way tube, 4-way tube, multiwall plates, PCR plates, Index sorting.

Flow cytometer (Cytoflex S):

Lasers - 488 nm, 50 mW (blue), 638 nm, 50 mW (red), 405 nm, 80 mW (violet), 561 nm, 30 mW (yellow-green)

Fluorescent parameters – 450/45 nm, 525/40 nm, 585/42 nm, 610/20 nm, 660/10, 690/ 50 nm, 712/25 nm, 780/60.

Side scattering and fluorescence – avalanche photodiodes

Blue laser side scattering (SSC) resolution - 300 nm.

Violet laser side scattering resolution (Violet SSC) - 200 nm.

Sensitivity – FITC - 30 MESF, PE - 10 MESF.

Sample containers – 5 ml, 1.5 ml, and 2 ml test tubes, standard 96-well plates with U, V-shaped and flat bottoms, with a plate sampler.